Yeast artificial Chromosome

Yeast artificial chromosomes are beginning to engineered chromosomes obtain from the DNA of the yeast and Saccharomyces cerevisiae which is then ligated into a bacterial plasmid. By placing large fragments based drug design of DNA and sequences can be cloned and physically survey using a process called chromosome walking. This is the procedure that was initially used for the Human Genetics, however due to stability issues, YACs were deserted for the use of Bacterial artificial chromosomes. The main components of a YAC are the ARS, telomeres and centromere from Saccharomyces cerevisiae. Additionally, selectable target genomics such as antibiotic resistance and a visible marker are utilized to select change yeast cells.

 

 

  • Bacterial artificial chromosome 
  • Autonomously replicating sequence(ARS)
  • Cosmid and Phasmid
  • Cloning Vector in Chromosome
  • Human artificial chromosome
  • Shuttle plasmid and Fosmid in YAC

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